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Home > Product > ELISA Kit > Rabbit Thioredoxin, TRX ELISA Kit

Rabbit Thioredoxin, TRX ELISA Kit

Rabbit Thioredoxin, TRX ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.18ng/L

Detection range: 10-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product Summary

Size: 48T,96T

Sensitivity: 5.18ng/L

Detection range: 10-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product overview

Full product name

Thioredoxin

Code

E0138Rb

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.18ng/L

Detection range

10-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rabbit

Storage

2-8ºC

Assay time

1h 30m

Background

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (By similarity). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (By similarity).

UniProt accession

MASS(Da)

11,761

GeneID

100339119

Synonyms

Thioredoxin;Trx

Gene names

TXN

Research area

Others

Target protein

Trx

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0138Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.088 2.228 2.184
2.369
800ng/L 1.659 1.585 1.54
1.51
400ng/L 1.006 1.018 0.973
1.03
200ng/L 0.738 0.761 0.717
0.784
100ng/L 0.485 0.446 0.401
0.407
0ng/L 0.054 0.045 0
0.035

Precision

We measured random samples of E0138Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 70.02 5.38 7.68
Intra-Assay 2 18 502.55 33.48 6.66
Intra-Assay 3 18 676.89 30.84 4.56

Protocols

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  • Size

    Code

    Price

  • 48T

    E0138Rb

    $320.00

  • 96T

    E0138Rb

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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