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Home > Product > ELISA Kit > Human Rho Guanine Nucleotide Exchange Factor 2, ARHGEF2 ELISA Kit

Human Rho Guanine Nucleotide Exchange Factor 2, ARHGEF2 ELISA Kit

Human Rho Guanine Nucleotide Exchange Factor 2, ARHGEF2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 8.03ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 8.03ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Rho Guanine Nucleotide Exchange Factor 2

Code

E3235Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

8.03ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed: 9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation (PubMed: 28453519). Involved in the migration of precerebellar neurons (By similarity).

UniProt accession

MASS(Da)

111,543

GeneID

9181

Synonyms

ARHGEF 2;ARHGEF2;GEF-H1;Guanine nucleotide exchange factor H1;Microtubule-regulated Rho-GEF;Proliferating cell nucleolar antigen p40;Rho guanine nucleotide exchange factor 2

Gene names

ARHGEF2

Research area

Others

Target protein

ARHGEF2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3235Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.224 2.174 2.126
2.125
800ng/L 1.282 1.241 1.193
1.201
400ng/L 0.598 0.644 0.595
0.69
200ng/L 0.298 0.369 0.32
0.439
100ng/L 0.17 0.153 0.104
0.136
0ng/L 0.044 0.049 0
0.054

Precision

We measured random samples of E3235Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 968.1 20.48 2.1
Intra-Assay 2 18 726.1 37.77 5.2
Intra-Assay 3 18 448.6 25.92 5.8

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E3235Hu

    $320.00

  • 96T

    E3235Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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