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Home > Product > ELISA Kit > Sheep Macrophage Migration Inhibitory Factor, MIF ELISA Kit

Sheep Macrophage Migration Inhibitory Factor, MIF ELISA Kit

Sheep Macrophage Migration Inhibitory Factor, MIF ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.281ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product Summary

Size: 48T,96T

Sensitivity: 0.281ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product overview

Full product name

Macrophage Migration Inhibitory Factor

Code

E0140Sh

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.281ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Sheep

Storage

2-8ºC

Assay time

1h 30m

Background

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity).

UniProt accession

MASS(Da)

12,343

GeneID

780466

Synonyms

L-dopachrome isomerase;L-dopachrome tautomerase;Macrophage migration inhibitory factor;MIF;Phenylpyruvate tautomerase

Gene names

MIF

Research area

Immunology

Target protein

MIF

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0140Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.458 2.494 2.459
2.529
4ng/ml 1.476 1.416 1.382
1.356
2ng/ml 0.916 0.909 0.875
0.902
1ng/ml 0.381 0.379 0.345
0.378
0.5ng/ml 0.281 0.238 0.203
0.195
0ng/ml 0.041 0.034 0
0.028

Precision

We measured random samples of E0140Sh within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.52 0.08 5.27
Intra-Assay 2 18 4.11 0.14 3.33
Intra-Assay 3 18 2.92 0.15 5.03

Protocols

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  • Size

    Code

    Price

  • 48T

    E0140Sh

    $320.00

  • 96T

    E0140Sh

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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