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Home > Product > ELISA Kit > Sheep Insulin-like Growth Factor 2, IGF2 ELISA Kit

Sheep Insulin-like Growth Factor 2, IGF2 ELISA Kit

Sheep Insulin-like Growth Factor 2, IGF2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.68ng/mL

Detection range: 6-1800ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product Summary

Size: 48T,96T

Sensitivity: 2.68ng/mL

Detection range: 6-1800ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product overview

Full product name

Insulin-like growth factor 2

Code

E0170Sh

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.68ng/mL

Detection range

6-1800ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Sheep

Storage

2-8ºC

Assay time

1h 30m

Background

The insulin-like growth factors possess growth-promoting activity (By similarity). Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver. Acts as a ligand for integrin which is required for IGF2 signaling. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (By similarity). Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate (By similarity). Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

UniProt accession

MASS(Da)

19,616

GeneID

443325

Synonyms

IGF2;IGF-II;Insulin-like growth factor II;Preptin]

Gene names

IGF2

Research area

Signal Transduction; Cancer; Developmental Biology

Target protein

IGF2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0170Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
960ng/mL 2.077 2.116 2.069
2.155
480ng/mL 0.92 1.047 1
1.175
240ng/mL 0.422 0.485 0.437
0.547
120ng/mL 0.272 0.265 0.217
0.257
60ng/mL 0.117 0.112 0.064
0.107
0ng/mL 0.055 0.048 0
0.041

Precision

We measured random samples of E0170Sh within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 544.23 22.02 4.05
Intra-Assay 2 18 662.06 15.45 2.33
Intra-Assay 3 18 218.34 9.93 4.55

Protocols

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  • Size

    Code

    Price

  • 48T

    E0170Sh

    $320.00

  • 96T

    E0170Sh

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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