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Home > Product > ELISA Kit > Sheep Interleukin-12 Subunit Beta, IL12B ELISA Kit

Sheep Interleukin-12 Subunit Beta, IL12B ELISA Kit

Sheep Interleukin-12 Subunit Beta, IL12B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.43ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product Summary

Size: 48T,96T

Sensitivity: 7.43ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product overview

Full product name

Interleukin-12 subunit beta

Code

E0171Sh

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.43ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Sheep

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis (By similarity).

UniProt accession

MASS(Da)

36,937

GeneID

443472

Synonyms

CLMF p40;Cytotoxic lymphocyte maturation factor 40 kDa subunit;IL-12;IL-12 subunit p40;IL12B;IL-12B;Interleukin-12 subunit beta

Gene names

IL12B

Research area

Immunology

Target protein

IL12B

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0171Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.195 2.141 2.093
2.086
800ng/L 1.373 1.461 1.413
1.548
400ng/L 0.894 0.859 0.811
0.824
200ng/L 0.539 0.522 0.475
0.505
100ng/L 0.251 0.248 0.201
0.245
0ng/L 0.057 0.048 0
0.038

Precision

We measured random samples of E0171Sh within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 394.57 20.14 5.1
Intra-Assay 2 18 1067.04 42.36 3.97
Intra-Assay 3 18 247.39 14.67 5.93

Protocols

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  • Size

    Code

    Price

  • 48T

    E0171Sh

    $320.00

  • 96T

    E0171Sh

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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