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Home > Product > ELISA Kit > Human Cyclin-dependent Kinase Inhibitor 1, CDKN1A ELISA Kit

Human Cyclin-dependent Kinase Inhibitor 1, CDKN1A ELISA Kit

Human Cyclin-dependent Kinase Inhibitor 1, CDKN1A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.94ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.94ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cyclin-Dependent Kinase Inhibitor 1

Code

E3243Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.94ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed: 11595739). Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed: 9106657).

UniProt accession

MASS(Da)

18,119

GeneID

1026

Synonyms

CDK-interacting protein 1;CDKN1A;Cyclin-dependent kinase inhibitor 1;MDA-6;Melanoma differentiation-associated protein 6;p21

Gene names

CDKN1A

Research area

Others

Target protein

CDKN1A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3243Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.487 2.443 2.41
2.399
1200ng/L 1.333 1.336 1.303
1.339
600ng/L 0.95 0.838 0.805
0.725
300ng/L 0.383 0.42 0.386
0.456
150ng/L 0.283 0.259 0.225
0.234
0ng/L 0.033 0.033 0
0.034

Precision

We measured random samples of E3243Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1138.6 36.83 3.2
Intra-Assay 2 18 365.7 23.51 6.4
Intra-Assay 3 18 745.1 33.08 4.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E3243Hu

    $320.00

  • 96T

    E3243Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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