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Home > Product > ELISA Kit > Rat Bone Morphogenetic Protein 4, BMP-4 ELISA Kit

Rat Bone Morphogenetic Protein 4, BMP-4 ELISA Kit

Rat Bone Morphogenetic Protein 4, BMP-4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.01ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 1.01ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Bone morphogenetic protein 4

Code

E0266Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.01ng/L

Detection range

2-600ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis (By similarity). Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical BMP pathways such as ERK/MAP kinase, PI3K/Akt, or SRC cascades. For example, induces SRC phosphorylation which, in turn, activates VEGFR2, leading to an angiogenic response (By similarity).

UniProt accession

MASS(Da)

46,541

GeneID

25296

Synonyms

BMP-2B;BMP-4;BOMPR4A;Bone morphogenetic protein 2B;Bone morphogenetic protein 4

Gene names

Bmp4

Research area

Cardiovascular; Signal Transduction; Cancer; Developmental Biology

Target protein

BMP-4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0266Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
320ng/L 2.171 2.165 2.122
2.159
160ng/L 1.66 1.601 1.558
1.542
80ng/L 0.988 1.015 0.972
1.043
40ng/L 0.625 0.656 0.613
0.687
20ng/L 0.486 0.478 0.435
0.471
0ng/L 0.06 0.043 0
0.026

Precision

We measured random samples of E0266Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 52.87 3.79 7.16
Intra-Assay 2 18 128.2 4.29 3.34
Intra-Assay 3 18 19.14 1.17 6.12

Protocols

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  • Size

    Code

    Price

  • 48T

    E0266Ra

    $320.00

  • 96T

    E0266Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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