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Home > Product > ELISA Kit > Rat Bone Morphogenetic Protein 6, BMP-6 ELISA Kit

Rat Bone Morphogenetic Protein 6, BMP-6 ELISA Kit

Rat Bone Morphogenetic Protein 6, BMP-6 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.44pg/ml

Detection range: 5-1000pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 2.44pg/ml

Detection range: 5-1000pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Bone morphogenetic protein 6

Code

E0277Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.44pg/ml

Detection range

5-1000pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes including cartilage and bone formation (By similarity). Plays also an important role in the regulation of iron metabolism by acting as a ligand for hemojuvelin/HJV (By similarity). Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2B. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target. Can also signal through non-canonical pathway such as TAZ-Hippo signaling cascade to modulate VEGF signaling by regulating VEGFR2 expression (By similarity).

UniProt accession

MASS(Da)

56,223

GeneID

25644

Synonyms

BMP-6;Bone morphogenetic protein 6;VG-1-related protein;VGR;VGR-1

Gene names

Bmp6

Research area

Signal Transduction; Cell Biology

Target protein

BMP-6

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0277Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
720pg/ml 2.135 2.138 2.083
2.141
360pg/ml 1.162 1.158 1.103
1.153
180pg/ml 0.636 0.727 0.671
0.817
90pg/ml 0.412 0.437 0.382
0.462
45pg/ml 0.233 0.249 0.194
0.266
0pg/ml 0.051 0.055 0
0.059

Precision

We measured random samples of E0277Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 87.81 5.1 5.8
Intra-Assay 2 18 398.91 11.56 2.9
Intra-Assay 3 18 206.17 11.84 5.74

Protocols

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  • Size

    Code

    Price

  • 48T

    E0277Ra

    $320.00

  • 96T

    E0277Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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