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Home > Product > ELISA Kit > Rat Alpha-fetoprotein, AFP ELISA Kit

Rat Alpha-fetoprotein, AFP ELISA Kit

Rat Alpha-fetoprotein, AFP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.004ng/ml

Detection range: 0.01-3ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.004ng/ml

Detection range: 0.01-3ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Alpha-fetoprotein

Code

E0332Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.004ng/ml

Detection range

0.01-3ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Binds estrogens, fatty acids and metals.

UniProt accession

MASS(Da)

68,386

GeneID

24177

Synonyms

AFP;AFPD;Alpha-1-fetoprotein;Alpha-fetoglobulin;Alpha-fetoprotein;FETA

Gene names

Afp

Research area

Cardiovascular; Signal Transduction; Cancer

Target protein

AFP

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0332Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1.6ng/ml 1.876 1.953 1.895
2.03
0.8ng/ml 0.97 0.935 0.877
0.899
0.4ng/ml 0.608 0.587 0.529
0.566
0.2ng/ml 0.245 0.25 0.192
0.256
0.1ng/ml 0.161 0.118 0.06
0.075
0ng/ml 0.055 0.058 0
0.061

Precision

We measured random samples of E0332Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 0.39 0.02 5.63
Intra-Assay 2 18 1.13 0.03 2.4
Intra-Assay 3 18 0.9 0.05 5.41

Protocols

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  • Size

    Code

    Price

  • 48T

    E0332Ra

    $320.00

  • 96T

    E0332Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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