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Home > Product > ELISA Kit > Human Tar DNA Binding Protein 43, TDP-43 ELISA Kit

Human Tar DNA Binding Protein 43, TDP-43 ELISA Kit

Human Tar DNA Binding Protein 43, TDP-43 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.49ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.49ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

TAR DNA binding protein 43

Code

E0333Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.49ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed:23519609). Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed:23519609, PubMed:24240615, PubMed:24464995). In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed:21358640, PubMed:29438978). Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed:28794432). Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening (PubMed:30520513). In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed:23398327, PubMed:19765185). Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed:30464263). Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed:27123980). Negatively regulates the expression of CDK6 (PubMed:19760257). Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed:25678563).

UniProt accession

MASS(Da)

44,740

GeneID

23435

Synonyms

ALS10;TAR DNA-binding protein 43;TARDBP;TDP43;TDP-43

Gene names

TARDBP

Research area

Microbiology; Neuroscience; Epigenetics and Nuclear Signaling

Target protein

TARDBP

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0333Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000ng/L 2.246 2.296 2.249
2.346
2000ng/L 1.671 1.615 1.568
1.559
1000ng/L 1.111 1.075 1.028
1.038
500ng/L 0.7 0.735 0.689
0.77
250ng/L 0.469 0.447 0.4
0.425
0ng/L 0.043 0.047 0
0.051

Precision

We measured random samples of E0333Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 826.95 59.37 7.18
Intra-Assay 2 18 1650.56 78.85 4.78
Intra-Assay 3 18 423.6 33.83 7.99

Protocols

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  • Size

    Code

    Price

  • 48T

    E0333Hu

    $320.00

  • 96T

    E0333Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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