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Home > Product > ELISA Kit > Rabbit Podocalyxin, PCX ELISA Kit

Rabbit Podocalyxin, PCX ELISA Kit

Rabbit Podocalyxin, PCX ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.046ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product Summary

Size: 48T,96T

Sensitivity: 0.046ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product overview

Full product name

Podocalyxin

Code

E0395Rb

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.046ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rabbit

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

UniProt accession

MASS(Da)

57,041

GeneID

100009552

Synonyms

PC;PCLP1;PCLP-1;PCX;Podocalyxin;Podocalyxin-like protein 1

Gene names

PODXL

Research area

Signal Transduction; Stem Cells; Cancer; Cardiovascular

Target protein

PCX

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0395Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.059 2.124 2.081
2.189
12ng/ml 1.401 1.277 1.234
1.152
6ng/ml 0.658 0.718 0.676
0.779
3ng/ml 0.425 0.413 0.37
0.401
1.5ng/ml 0.23 0.245 0.202
0.259
0ng/ml 0.048 0.043 0
0.038

Precision

We measured random samples of E0395Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.45 0.18 5.32
Intra-Assay 2 18 7.48 0.39 5.17
Intra-Assay 3 18 14 0.51 3.66

Protocols

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  • Size

    Code

    Price

  • 48T

    E0395Rb

    $320.00

  • 96T

    E0395Rb

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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