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Home > Product > ELISA Kit > Porcine Catechol-O-methyltransferase, COMT ELISA Kit

Porcine Catechol-O-methyltransferase, COMT ELISA Kit

Porcine Catechol-O-methyltransferase, COMT ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.11ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 0.11ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Catechol-O-Methyltransferase

Code

E0445Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.11ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

UniProt accession

MASS(Da)

20,587

GeneID

100155530

Synonyms

Catechol O-methyltransferase;COMT;Fragment

Gene names

COMT

Research area

Neuroscience; Signal Transduction; Metabolism

Target protein

COMT

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0445Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.398 2.516 2.471
2.635
20ng/ml 1.598 1.513 1.468
1.429
10ng/ml 0.886 0.802 0.757
0.717
5ng/ml 0.411 0.465 0.42
0.519
2.5ng/ml 0.194 0.234 0.189
0.273
0ng/ml 0.049 0.045 0
0.041

Precision

We measured random samples of E0445Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 13.19 0.57 4.36
Intra-Assay 2 18 18.33 0.59 3.2
Intra-Assay 3 18 4.55 0.2 4.34

Protocols

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  • Size

    Code

    Price

  • 48T

    E0445Po

    $320.00

  • 96T

    E0445Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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