Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Porcine Trypsin, TRYPSIN ELISA Kit

Porcine Trypsin, TRYPSIN ELISA Kit

Porcine Trypsin, TRYPSIN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.52ng/ml

Detection range: 1-350ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 0.52ng/ml

Detection range: 1-350ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Trypsin

Code

E0475Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.52ng/ml

Detection range

1-350ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Trypsin (EC 3.4.21.4) is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyzes proteins. Trypsin is formed in the small intestine when its proenzyme form, the trypsinogen produced by the pancreas, is activated. Trypsin cuts peptide chains mainly at the carboxyl side of the amino acids lysine or arginine. It is used for numerous biotechnological processes. The process is commonly referred to as trypsin proteolysis or trypsinization, and proteins that have been digested/treated with trypsin are said to have been trypsinized.

UniProt accession

MASS(Da)

24,409

GeneID

548509

Synonyms

trp1;try1;trypsin

Gene names

trypsin

Research area

Signal Transduction; Metabolism

Target protein

trypsin

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0475Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
200ng/ml 2.346 2.292 2.257
2.238
100ng/ml 1.291 1.354 1.318
1.416
50ng/ml 0.705 0.635 0.599
0.565
25ng/ml 0.338 0.392 0.356
0.446
12.5ng/ml 0.144 0.143 0.107
0.141
0ng/ml 0.028 0.036 0
0.043

Precision

We measured random samples of E0475Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 46.98 2.28 4.86
Intra-Assay 2 18 65.53 2.93 4.47
Intra-Assay 3 18 119.21 4.02 3.38

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E0475Po

    $320.00

  • 96T

    E0475Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up