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Home > Product > ELISA Kit > Rat Neurotrophin 3, NT-3 ELISA Kit

Rat Neurotrophin 3, NT-3 ELISA Kit

Rat Neurotrophin 3, NT-3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.21ng/ml

Detection range: 0.5-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.21ng/ml

Detection range: 0.5-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Neurotrophin 3

Code

E0543Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.21ng/ml

Detection range

0.5-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Neurotrophin-3 is a protein that in humans is encoded by the NTF3 gene. The protein encoded by this gene, NT-3, is a neurotrophic factor in the NGF (Nerve Growth Factor) family of neurotrophins. It is a protein growth factor which has activity on certain neurons of the peripheral and central nervous system; it helps to support the survival and differentiation of existing neurons, and encourages the growth and differentiation of new neurons and synapses. NT-3 was the third neurotrophic factor to be characterized, after nerve growth factor (NGF) and BDNF (Brain Derived Neurotrophic Factor).

UniProt accession

MASS(Da)

29,645

GeneID

81737

Synonyms

HDNF;Nerve growth factor 2;Neurotrophic factor;Neurotrophin-3;NGF-2;NT-3

Gene names

Ntf3

Research area

Neuroscience; Cardiovascular; Metabolism

Target protein

NT-3

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0543Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.234 2.215 2.161
2.196
16ng/ml 1.328 1.358 1.303
1.387
8ng/ml 0.763 0.8 0.746
0.838
4ng/ml 0.475 0.454 0.399
0.432
2ng/ml 0.23 0.268 0.214
0.306
0ng/ml 0.07 0.054 0
0.038

Precision

We measured random samples of E0543Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 19.83 0.78 3.94
Intra-Assay 2 18 11.77 0.73 6.17
Intra-Assay 3 18 6.51 0.44 6.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E0543Ra

    $320.00

  • 96T

    E0543Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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