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Home > Product > ELISA Kit > Human Neurogenic Locus Notch Homolog Protein 2, NOTCH2 ELISA Kit

Human Neurogenic Locus Notch Homolog Protein 2, NOTCH2 ELISA Kit

Human Neurogenic Locus Notch Homolog Protein 2, NOTCH2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Neurogenic Locus Notch Homolog Protein 2

Code

E3262Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.26ng/ml

Detection range

0.5-100ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed: 21378985, PubMed: 21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed: 29149593). Positively regulates self-renewal of liver cancer cells (PubMed: 25985737).

UniProt accession

MASS(Da)

265,405

GeneID

4853

Synonyms

hN2;N2ECD;N2ICD;Neurogenic locus notch homolog protein 2;NOTCH 2;Notch 2 extracellular truncation;Notch 2 intracellular domain;NOTCH2

Gene names

NOTCH2

Research area

Others

Target protein

NOTCH2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3262Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
64ng/ml 2.079 2.234 2.192
2.388
32ng/ml 1.496 1.563 1.521
1.629
16ng/ml 1.082 1.103 1.061
1.123
8ng/ml 0.643 0.637 0.596
0.632
4ng/ml 0.433 0.431 0.39
0.43
0ng/ml 0.048 0.041 0
0.035

Precision

We measured random samples of E3262Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 27.2 1.21 4.4
Intra-Assay 2 18 18 1.2 6.7
Intra-Assay 3 18 5.9 0.43 7.3

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3262Hu

    $320.00

  • 96T

    E3262Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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