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Home > Product > ELISA Kit > Rat Chromogranin-A, CGA ELISA Kit

Rat Chromogranin-A, CGA ELISA Kit

Rat Chromogranin-A, CGA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.93ng/ml

Detection range: 2-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.93ng/ml

Detection range: 2-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Chromogranin-A

Code

E0557Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.93ng/ml

Detection range

2-600ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Strongly inhibits glucose induced insulin release from the pancreas. Catestatin inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Can induce mast cell migration, degranulation and production of cytokines and chemokines. Serpinin regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation (By similarity). Serpinin and pGlu-serpinin can enhance both myocardial contractility (inotropy) and relaxation (lusitropy) and this cardio-stimulation requires a beta 1-adrenergic receptor/adenylate cyclase/cAMP/PKA pathway (PubMed:22459152).

UniProt accession

MASS(Da)

52,024

GeneID

24258

Synonyms

Beta-granin;Catestatin;CgA;Chromogranin-A;GE-25;Pancreastatin;p-Glu serpinin precursor];Serpinin;Serpinin-RRG;WE-14

Gene names

Chga

Research area

Signal Transduction; Neuroscience; Cancer; Metabolism

Target protein

CgA

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0557Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
320ng/ml 2.143 2.124 2.063
2.105
160ng/ml 1.382 1.363 1.303
1.345
80ng/ml 0.809 0.789 0.729
0.769
40ng/ml 0.423 0.427 0.367
0.431
20ng/ml 0.247 0.26 0.2
0.274
0ng/ml 0.048 0.06 0
0.073

Precision

We measured random samples of E0557Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 212.69 10.01 4.71
Intra-Assay 2 18 120.26 6.84 5.69
Intra-Assay 3 18 54.4 3 5.52

Protocols

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  • Size

    Code

    Price

  • 48T

    E0557Ra

    $320.00

  • 96T

    E0557Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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