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Product > Recombinant proteins > Recombinant Protein A (C-6His)
Recombinant Protein A (C-6His)
Recombinant Protein A (C-6His)
Product Summary
Size:
10 mg
Purity: > 97 % by SDS-PAGE analyses.
Source: Escherichia coli.
Product Summary
Size:
10 mg
Purity: > 97 % by SDS-PAGE analyses.
Source: Escherichia coli.
Product overview
Product name
Recombinant Protein A (C-6His)
Code
CD00149
Endotoxin
Less than 0.1 EU/μg of Protein A, His as determined by LAL method.
Molecular Weight
Approximately 34.7 kDa with 6×His-tag on C-terminus, a single non-glycosylated polypeptide chain containing 306 amino acids.
Formulation
Lyophilized with no additive.
Reconstitution
Dissolve in distilled water or saline.
Background
The Protein A molecule contains four high-affinity (Ka =108/mole) binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit. Optimal binding occurs a pH 8.2, although binding is also good at neutral or physiological conditions (pH 7.0-7.6).
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