Recombinant Murine Stem Cell Factor

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Home > Product > Recombinant proteins > Recombinant Murine Stem Cell Factor

Recombinant Murine Stem Cell Factor

Product Summary

Size: 2ug

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product Summary

Size: 2ug

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product overview

Product name

Recombinant Murine Stem Cell Factor

Code

CD0175

Biological activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.

Endotoxin

Less than 1 EU/μg of rMuSCF as determined by LAL method.

Synonyms

SCF

Sequence

MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Uniprot Accession

NP_038626.1

Molecular Weight

Approximately 18.4 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.

Background

Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane It is required for normal hematopoietic function and plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. It also promotes mast cell adhesion, migration, proliferation, and survival.

Storage

-20ºC

Research area

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Datasheet

Size

Code

Price

2ug

CD0175

$128.00

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Lead time: Within two weeks

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