Product name
Recombinant Human IL-17F protein
Code
CD01549
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of Mouse NIH/3T3 cells is less than 20 ng/ml, corresponding to a specific activity of > 5.0 × 104 IU/mg.
Endotoxin
Less than 1 EU/μg of rHuIL-17F as determined by LAL method.
Synonyms
IL-17F,CANDF6, IL17F, ML-1, ML1, Human
Sequence
MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ
Uniprot Accession
Molecular Weight
Approximately 30.1 kDa, a disulfide-linked homodimer of two 134 amino acid polypeptide chains.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2, with trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Human Interleukin-17F (IL-17F) is encoded by the IL17F gene located on the chromosome 6 and belongs to the IL-17 family which contains IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17F that shares 50 % homologous of crystal structure to IL-17A and is expressed by activated T cells, has the functions of stimulating production of other cytokines (such as IL-6, IL-8 and granulocyte colony-stimulating factor) and proliferation of PBMC and T-cell, regulating cartilage matrix turnover, and inhibiting angiogenesis.
Storage
-20ºC
Research area
Immunology
