Catenin-Beta Monoclonal Antibody(4F2)

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Blood coagulation
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Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
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Home > Product > Antibody > Catenin-Beta Monoclonal Antibody(4F2)

Catenin-Beta Monoclonal Antibody(4F2)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: WB, IF, ICC, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: WB, IF, ICC, IHC-p

Product overview

Full product name

Catenin beta-1

Code

BT-MCA0016

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Recombinant Protein of Catenin-Beta

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

N/A

Gene ID

Human: 1499

Synonyms

CTNNB1; CTNNB; OK; SW-cl.35; Catenin beta-1; Beta-catenin

Research area

Signal transduction; Cancer; Epigenetics and nuclear signaling; Stem cells; Neuroscience

Target protein

CTNNB1

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:200-500
WB	1:1000-2000

Images

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Catenin-Beta Monoclonal antibody(4F2) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1.Catenin-Beta Monoclonal antibody(4F2) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-stomach-cancer tissue. 1.Catenin-Beta Monoclonal antibody(4F2)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-spleen tissue. 1.Catenin-Beta Monoclonal antibody(4F2)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1) Hela, 2) 293T, 3) Mouse Liver Tissue, 4) Rat Liver Tissue using Catenin-Beta Monoclonal antibody.

Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using Catenin-Beta Monoclonal antibody.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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