Fibronectin Monoclonal Antibody(M9)

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Home > Product > Antibody > Fibronectin Monoclonal Antibody(M9)

Fibronectin Monoclonal Antibody(M9)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC,

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: WB, IHC-p, IF, ICC,

Product overview

Full product name

Fibronectin

Code

BT-MCA0036

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of Fibronectin

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

262607

Gene ID

Human: 2335; Mouse: 14268

Synonyms

FN1; FN; Fibronectin; FN; Cold-insoluble globulin; CIG

Research area

Cancer; Signal transduction; Developmental biology; Stem cells; Cardiovascular

Target protein

FN1

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:50-300
WB	1:1000-2000

Images

Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1.Fibronectin Monoclonal antibody(M9) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1.Fibronectin Monoclonal antibody(M9) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-appendix tissue. 1.Fibronectin Monoclonal antibody(M9)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-spleen tissue. 1.Fibronectin Monoclonal antibody(M9)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of Hela diluted at 1:2000.

Immunohistochemical analysis of paraffin-embedded Human kidney.1.Antibody was diluted at 1:100(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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