Immunofluorescence analysis of Hela cell. c-Fos Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). Alpha-SMA Monoclonal antibody(6A12)(green) was diluted at 1:200(4°C overnight).
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1.Alpha-SMA Monoclonal antibody(6A12) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.Alpha-SMA Monoclonal antibody(6A12) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1.Alpha-SMA Monoclonal antibody(6A12) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-liver tissue. 1.Alpha-SMA Monoclonal antibody(6A12)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-liver tissue. 1.Alpha-SMA Monoclonal antibody(6A12)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-liver tissue. 1.Alpha-SMA Monoclonal antibody(6A12)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of Hela, 3T3 and Rat Brain using Alpha-SMA Monoclonal antibody.
Immunohistochemical analysis of paraffin-embedded Mouse Cecal Tissue using Alpha-SMA Monoclonal antibody.
Immunohistochemical analysis of paraffin-embedded Human testis.1.Antibody was diluted at 1:200(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human testis.1.Antibody was diluted at 1:200(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human testis.1.Antibody was diluted at 1:200(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
