Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1.CD4 Monoclonal antibody(11A1) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CD4 Monoclonal antibody(11A1) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1.CD4 Monoclonal antibody(11A1) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Mouse-colon tissue. 1.CD4 Monoclonal antibody(11A1)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-lung tissue. 1.CD4 Monoclonal antibody(11A1)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of paraffin-embedded Mouse Colonic tissue
Immunofluorescence analysis of paraffin-embedded Mouse Colonic tissue
Immunofluorescence analysis of paraffin-embedded Mouse Colonic tissue
Immunohistochemical analysis of paraffin-embedded Human Amygdala.1.Antibody was diluted at 1:400(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Amygdala.1.Antibody was diluted at 1:400(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Amygdala.1.Antibody was diluted at 1:400(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human pancreas.1.Antibody was diluted at 1:200(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human pancreas.1.Antibody was diluted at 1:200(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human pancreas.1.Antibody was diluted at 1:200(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
