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Home > Product > Antibody > PTEN Monoclonal Antibody

PTEN Monoclonal Antibody

PTEN Monoclonal Antibody

Product Summary

Size: 50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse

Applications: WB, IF, ICC, FCM, ELISA

Product Summary

Size: 50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse

Applications: WB, IF, ICC, FCM, ELISA

Product overview

Full product name

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

Code

BT-MCA1072

Host

Mouse

Isotype

IgG

Size

50ul,100ul

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of PTEN expressed in E. Coli.

Purification

Affinity purification

Concentration

1 mg/ml

Formulation

Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.

Storage

-20°C for one year

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate ener

Uniprot accession

Molecular weight

N/A

Gene ID

Human: 5728; Mouse: 19211

Synonyms

PTEN; MMAC1; TEP1; Phosphatidylinositol 3; 4; 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog

Research area

Signal transduction; Metabolism; Cancer; Epigenetics and nuclear signaling; Cell biology

Target protein

PTEN

Recommended dilution

Application Dilution
ELISA 1:10000
FC 1:200 - 1:400
IF 1:200 - 1:1000
WB 1:500 - 1:2000

Images

Western Blot analysis using PTEN Monoclonal antibody against HeLa (1) and NIH/3T3 (2) cell lysate.
Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using PTEN Monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of Hela cells using PTEN Monoclonal antibody (right) and negative control (left).

Protocols

Customer reviews and Q&As

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Product size

Price

  • 50ul

    $180.00

  • 100ul

    $300.00

Add to Cart

Lead time: Within one week

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