14-3-3 theta/tau(Phospho Ser232) Polyclonal Antibody

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Home > Product > Antibody > 14-3-3 theta/tau(Phospho Ser232) Polyclonal Antibody

14-3-3 theta/tau(Phospho Ser232) Polyclonal Antibody

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

14-3-3 protein theta

Code

BT-AP00027

Host

Rabbit

Isotype

IgG

Size

100ul,50ul,20ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human 14-3-3 thet/tau around the phosphorylation site of Ser232. AA range:196-245

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

27764

Gene ID

Human: 5350; Mouse: 22630; Rat: 25577

Synonyms

YWHAQ; YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1

Research area

Neuroscience; Signal Transduction; Stem Cells

Target protein

YWHAQ

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 5000

Images

Immunofluorescence analysis of Hela cell. 14-3-3 θ/τ (phospho Ser232) Polyclonal Antibody(Green) was diluted at 1:200(4°C overnight). (Red) was diluted at 1:200(4°C overnight).

Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human pancreas, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of 293T MOUSE-BRAIN using p-14-3-3 θ/τ (S232) antibody. Antibody was diluted at 1:500

Western blot analysis of lysates from HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

100ul

$275.00
50ul

$180.00
20ul

$108.00

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Lead time: Within one week

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