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Home > Product > Antibody > eIF2 alpha(Phospho Ser51) Polyclonal Antibody

eIF2 alpha(Phospho Ser51) Polyclonal Antibody

eIF2 alpha(Phospho Ser51) Polyclonal Antibody

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: IF, ICC, WB, IHC-p, ELISA

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IF, ICC, WB, IHC-p, ELISA

Product overview

Full product name

Eukaryotic translation initiation factor 2 subunit 1

Code

BT-AP00593

Host

Rabbit

Isotype

IgG

Size

100ul,50ul,20ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human eIF2 alpha around the phosphorylation site of Ser51. AA range:21-70

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987

Uniprot accession

Molecular weight

36112

Gene ID

Human: 1965; Mouse: 13665; Rat: 54318

Synonyms

Eukaryotic translation initiation factor 2 subunit 1; EIF2S1; EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha

Research area

Epigenetics and nuclear signaling

Target protein

EIF2S1

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC-p 1: 100 - 1: 300
IF 1: 50 - 1: 200
ELISA 1: 10000

Images

Immunofluorescence analysis of rat-heart tissue. 1,eIF2α (phospho Ser51) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-heart tissue. 1,eIF2α (phospho Ser51) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of lysates from 1) KB, 2) MCF-7 cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, Dylight 800 secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Tubulin β Monoclonal Antibody(5G3) was diluted at 1:5000 as loading control, 4°C overnight,Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1hour.
Western Blot analysis of various cells using Phospho-eIF2α (S51) Polyclonal Antibody diluted at 1:2000
Western Blot analysis of 293 cells using Phospho-eIF2α (S51) Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from K562 cells treated with IFN-alpha 1000U/ml 18h, using eIF2 alpha (Phospho-Ser51) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

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Product size

Price

  • 100ul

    $275.00

  • 50ul

    $180.00

  • 20ul

    $108.00

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Lead time: Within one week

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