Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > Antibody > FoxO3A(Phospho Ser253) Polyclonal Antibody

FoxO3A(Phospho Ser253) Polyclonal Antibody

FoxO3A(Phospho Ser253) Polyclonal Antibody

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Forkhead box protein O3

Code

BT-AP00726

Host

Rabbit

Isotype

IgG

Size

100ul,50ul,20ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human FKHRL1 around the phosphorylation site of Ser253. AA range:220-269

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

Uniprot accession

Molecular weight

71277

Gene ID

Human: 2309; Mouse: 56484

Synonyms

Forkhead box protein O3; FOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1

Research area

Epigenetics and nuclear signaling

Target protein

FOXO3

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC-p 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ICC 1: 200 - 1: 1000
ELISA 1: 20000

Images

Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FKHRL1 (Phospho-Ser253) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of HELA cells using Phospho-FoxO3A (S253) Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from LOVO cells treated with serum 20% 30', using FKHRL1 (Phospho-Ser253) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

Customer reviews and Q&As

No reviews
No reviews

Product size

Price

  • 100ul

    $275.00

  • 50ul

    $180.00

  • 20ul

    $108.00

Add to Cart

Lead time: Within one week

We are continuously updating our Antibody catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up