Immunofluorescence analysis of rat-spleen tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-spleen tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,FAS-L Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 beta (Phospho-Ser9) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:1000
Western Blot analysis of Hela cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:1000
Western blot analysis of lysates from HeLa cells treated with EGF, using GSK3 beta (Phospho-Ser9) Antibody. The lane on the right is blocked with the phospho peptide.
