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Home > Product > Antibody > TGF beta 1 Polyclonal Antibody

TGF beta 1 Polyclonal Antibody

TGF beta 1 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Transforming growth factor beta-1

Code

BT-AP08147

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human TGF beta1. AA range:336-385

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide| and is found in either a latent form composed of a mature peptide homodimer| a LAP homodimer| and a latent TGF-beta binding protein| or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation| differentiation and growth| and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene i

Uniprot accession

Molecular weight

44341

Gene ID

Human: 7040; Mouse: 21803; Rat: 59086

Synonyms

Transforming growth factor beta-1; TGFB1; TGFB; Transforming growth factor beta-1; TGF-beta-1

Research area

Signal transduction; Stem cells; Cancer; Metabolism; Cardiovascular

Target protein

TGFB1

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC-p 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ICC 1: 200 - 1: 1000
ELISA 1: 20000

Images

Immunofluorescence analysis of rat-lung tissue. 1,TGFβ1 Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. 1,TGFβ1 Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-spleen tissue. 1,TGFβ1 Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-spleen tissue. 1,TGFβ1 Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of mouse-lung tissue. 1,Survivin Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of mouse-lung tissue. 1,TGFβ1 Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemical analysis of paraffin-embedded Human Endometrium. 1, Antibody was diluted at 1:200(4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human Endometrium. 1, Antibody was diluted at 1:200(4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Endometrium. 1, Antibody was diluted at 1:200(4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,TGFβ1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TGF beta1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 1) Mouse Brain, 2) HT29 , 3) HepG2 cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Monoclonal Antibody(2B8) antibody was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.
Western Blot analysis of various cells using TGFβ1 Polyclonal Antibody diluted at 1:2000
Western Blot analysis of MCF7 cells using TGFβ1 Polyclonal Antibody diluted at 1:2000
Western Blot analysis of mouse-brain using TGFβ1 Polyclonal Antibody. Antibody was diluted at 1:2000
Western blot analysis of lysates from HepG2 cells, using TGF beta1 Antibody. The lane on the right is blocked with the synthesized peptide.

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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