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Home > Product > Antibody > PKC theta(Phospho Thr538) Polyclonal Antibody

PKC theta(Phospho Thr538) Polyclonal Antibody

PKC theta(Phospho Thr538) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Protein kinase C theta type

Code

BT-AP13108

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human PKC thet around the phosphorylation site of Thr538. AA range:504-553

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.

Uniprot accession

Molecular weight

81865

Gene ID

Human: 5588; Mouse: 18761

Synonyms

Protein kinase C theta type; PRKCQ; PRKCT; Protein kinase C theta type; nPKC-theta

Research area

Cancer; Metabolism; Signal transduction

Target protein

PRKCQ

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC-p 1: 100 - 1: 300
ELISA 1: 5000

Images

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using PKC thet (Phospho-Thr538) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from Jurkat cells, using PKC thet (Phospho-Thr538) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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