RAP1A Monoclonal Antibody

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Home > Product > Antibody > RAP1A Monoclonal Antibody

RAP1A Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: FCM

Product overview

Code

BT-MCA3260

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human RAP1A expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

Uniprot accession

P62834:;

Molecular weight

43.2kDa

Gene ID

5906

Synonyms

RAP1;KREV1;KREV-1;SMGP21

Research area

Signal transduction

Target protein

RAP1A

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using RAP1A mAb against human RAP1A (AA: 28-180) recombinant protein. (Expected MW is 21 kDa)

Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.

Flow cytometric analysis of HeLa cells using RAP1A mouse mAb (green) and negative control (red).

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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