MAPK3 Monoclonal Antibody

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Home > Product > Antibody > MAPK3 Monoclonal Antibody

MAPK3 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat,Monkey

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA4719

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human MAPK3 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Uniprot accession

P27361:;

Molecular weight

44kDa

Gene ID

5595

Synonyms

ERK1;PRKM3;P44ERK1;P44MAPK;HS44KDAP;HUMKER1A;MGC20180

Research area

Neuroscience

Target protein

MAPK3

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using MAPK3 mAb against human MAPK3 (AA: 9-143) recombinant protein. (Expected MW is 40.8 kDa)

Western blot analysis using MAPK3 mouse mAb against Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using MAPK3 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MAPK3 mouse mAb with DAB staining.

Immunofluorescence analysis of NIH/3T3 cells using MAPK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of Hela cells using MAPK3 mouse mAb (blue) and negative control (red).

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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