CD82 Monoclonal Antibody

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Home > Product > Antibody > CD82 Monoclonal Antibody

CD82 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse

Applications: IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse

Applications: IHC,FCM

Product overview

Code

BT-MCA3368

Host

Mouse

Isotype

Mouse IgG2a

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CD82 (AA: extra 111-228) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Uniprot accession

P27701:;

Molecular weight

29.6kDa

Gene ID

3732

Synonyms

R2;4F9;C33;IA4;ST6;GR15;KAI1;SAR2;TSPAN27

Research area

Cancer,Epigenetics and nuclear signaling,Immunology

Target protein

CD82

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using CD82 mAb against human CD82 (AA: extra 111-228) recombinant protein. (Expected MW is 39.5 kDa)

Western blot analysis using CD82 mAb against HEK293-6e (1) and CD82 (AA: extra 111-228)-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis of NIH/3T3 cells using CD82 mouse mAb (green) and negative control (red).

Flow cytometric analysis of Hela cells using CD82 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using CD82 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded human lymph tissues using CD82 mouse mAb with DAB staining.

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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