UCHL1 Monoclonal Antibody

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Home > Product > Antibody > UCHL1 Monoclonal Antibody

UCHL1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA4030

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human UCHL1 (AA: 1-220) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.

Uniprot accession

P09936:;

Molecular weight

24.8kDa

Gene ID

7345

Synonyms

NDGOA;PARK5;PGP95;SPG79;PGP9.5;Uch-L1;HEL-117;PGP 9.5;HEL-S-53

Research area

Neuroscience

Target protein

UCHL1

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:50 - 1:200
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using UCHL1 mAb against human UCHL1 (AA: 1-220) recombinant protein. (Expected MW is 27.5 kDa)

Western blot analysis using UCHL1 mAb against HEK293-6e (1) and UCHL1 (AA: 1-220)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using UCHL1 mouse mAb against mouse brain (1), and rat brain (2) cell lysate.

Flow cytometric analysis of Hek293 cells using UCHL1 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using UCHL1 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded brain tissues using UCHL1 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using UCHL1 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using UCHL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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