KRT14 Monoclonal Antibody

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Home > Product > Antibody > KRT14 Monoclonal Antibody

KRT14 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Monkey

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Monkey

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA4413

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human KRT14 (AA: 115-472) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

Uniprot accession

P02533:;

Molecular weight

51.5kDa

Gene ID

3861

Synonyms

K14;NFJ;CK14;EBS3;EBS4

Research area

Signal Transduction

Target protein

KRT14

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using KRT14 mAb against human KRT14 (AA: 115-472) recombinant protein. (Expected MW is 43.9 kDa)

Western blot analysis using KRT14 mAb against HEK293-6e (1) and KRT14 (AA: 115-472)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using KRT14 mouse mAb against MCF-7 (1), Hela (2), A431 (3), SW480 (4), T47D (5) and Molt-4 (6) cell lysate.

Immunofluorescence analysis of Hela cells using KRT14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using KRT14 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded prostatic cancer tissues using KRT14 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded esophagus tissues using KRT14 mouse mAb with DAB staining.

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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