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Home > Product > Antibody > PTGS2 Monoclonal Antibody

PTGS2 Monoclonal Antibody

PTGS2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: ICC,FCM

Product overview

Code

BT-MCA4411

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human PTGS2 (AA: 18-207) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.

Uniprot accession

Molecular weight

69kDa

Gene ID

5743

Synonyms

COX2;COX-2;PHS-2;PGG/HS;PGHS-2;hCox-2;GRIPGHS

Research area

Immunology

Target protein

PTGS2

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
ICC 1:200 - 1:1000
FCM 1:200 - 1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using PTGS2 mAb against human PTGS2 (AA: 18-207) recombinant protein. (Expected MW is 47.8 kDa)
Western blot analysis using PTGS2 mAb against HEK293-6e (1) and human PTGS2 (AA: 18-207)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis of Hela cells using PTGS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using PTGS2 mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using PTGS2 mouse mAb (green) and negative control (red).
Flow cytometric analysis of HT-29 cells using PTGS2 mouse mAb (green) and negative control (red).

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

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