VCP Monoclonal Antibody

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Home > Product > Antibody > VCP Monoclonal Antibody

VCP Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,ICC,FCM

Product overview

Code

BT-MCA2873

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human VCP (AA:707-806) expressed in Mammalian system

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients.

Uniprot accession

P55072:;

Molecular weight

89.3kDa

Gene ID

7415

Synonyms

p97;TERA;CDC48;FTDALS6

Research area

Neuroscience

Target protein

VCP

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using VCP mAb against human VCP (AA:707-806) recombinant protein. (Expected MW is 42 kDa)

Western blot analysis using VCP mouse mAb against Hela (1), A549 (2), NIH/3T3 (3), Raw264.7 (4), SH-SY5Y (5), MCF-7 (6), and A431 (7) cell lysate.

Flow cytometric analysis of MOLT4 cells using VCP mouse mAb (green) and negative control (red).

Flow cytometric analysis of HL-60 cells using VCP mouse mAb (green) and negative control (red).

Flow cytometric analysis of THP-1 cells using VCP mouse mAb (green) and negative control (red).

Immunofluorescence analysis of Hela cells using VCP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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