ITGAM Monoclonal Antibody

Product

OneStep ELISA kit
Primacu ELISA kit
Antibodies
Biochemicals
Recombinant proteins

Service

Custom ELISA assay services
Bulk quote

Research area

Blood coagulation
Metabolism
Cancer
Microbiology
Cardiovascular
Neuroscience
Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
Tags & cell markers
Immunology

Resources

Protocols
FAQs
Product brochure
Promotion
Troubleshooting guides
About ELISAs

About us

Company profile
Worldwide distributors
Manufacturing service

Home > Product > Antibody > ITGAM Monoclonal Antibody

ITGAM Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: IHC,ICC,FCM

Product overview

Code

BT-MCA2471

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human ITGAM (AA: 623-728) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

P11215:;

Molecular weight

127.1kDa

Gene ID

3684

Synonyms

CR3A;MO1A;CD11B;MAC-1;MAC1A;SLEB6

Research area

Signal transduction,Stem cells,Neuroscience,Tags & cell markers,Immunology

Target protein

ITGAM

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using ITGAM mAb against human ITGAM (AA: 623-728) recombinant protein. (Expected MW is 37.5 kDa)

Western blot analysis using ITGAM mAb against HEK293-6e (1) and ITGAM (AA: 623-728)-hIgGFc transfected HEK293-6e (2) cell lysate.

Immunofluorescence analysis of Hela cells using ITGAM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of HL-60 cells using ITGAM mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded spleen tissues using ITGAM mouse mAb with DAB staining.

Protocols

> Protocol Booklet

Customer reviews and Q&As

No reviews

Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

We are continuously updating our Antibody catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.