SIGLEC8 Monoclonal Antibody

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Home > Product > Antibody > SIGLEC8 Monoclonal Antibody

SIGLEC8 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,FCM

Product overview

Code

BT-MCA4447

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human SIGLEC8 (AA: extra 17-216) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983])

Uniprot accession

Q9NYZ4:;

Molecular weight

54kDa

Gene ID

27181

Synonyms

SAF2;SIGLEC-8;SIGLEC8L

Research area

Immunology

Target protein

SIGLEC8

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using SIGLEC8 mAb against human SIGLEC8 (AA: extra 17-216) recombinant protein. (Expected MW is 25.3 kDa)

Western blot analysis using SIGLEC8 mAb against HEK293-6e (1) and SIGLEC8 (AA: extra 17-216)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using SIGLEC8 mouse mAb against mouse Liver (1), rat Liver (2)tissues lysate, MCF-7 (3), and HT-29 (4) cell lysate.

Flow cytometric analysis of Jurkat cells using SIGLEC8 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using SIGLEC8 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded rectum tissues using SIGLEC8 mouse mAb with DAB staining.

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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