G6D Monoclonal Antibody

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Home > Product > Antibody > G6D Monoclonal Antibody

G6D Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,ICC,FCM

Product overview

Code

BT-MCA4807

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human G6D (AA: 20-104) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

LY6G6D belongs to a cluster of leukocyte antigen-6 (LY6) genes located in the major histocompatibility complex (MHC) class III region on chromosome 6. Members of the LY6 superfamily typically contain 70 to 80 amino acids, including 8 to 10 cysteines. Most LY6 proteins are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor that is directly involved in signal transduction (Mallya et al., 2002 [PubMed 12079290]).[supplied by OMIM, Apr 2009]

Uniprot accession

O95868:;

Molecular weight

13.7kDa

Gene ID

58530

Synonyms

LY6G6D;NG25;LY6-D;MEGT1;C6orf23

Research area

Others

Target protein

G6D

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using G6D mAb against human G6D (AA: 20-104) recombinant protein. (Expected MW is 21.4 kDa)

Western blot analysis using G6D mAb against HEK293-6e (1) and G6D (AA: 20-104)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using G6D mouse mAb against Jurkat (1), MCF-7 (2), A549 (3),K562 (4),Hela (5),C6 (6),COS-7 (7),and NIH/3T3 (8) cell lysate.

Flow cytometric analysis of Jurkat cells using G6D mouse mAb (green) and negative control (red).

Immunofluorescence analysis of Hela cells using G6D mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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