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Home > Product > Antibody > CD1A Monoclonal Antibody

CD1A Monoclonal Antibody

CD1A Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA4471

Host

Mouse

Isotype

Mouse IgG2a

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CD1A (AA: 17-116) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot accession

Molecular weight

37kDa

Gene ID

909

Synonyms

R4;T6;CD1;FCB6;HTA1

Research area

Immunology

Target protein

CD1A

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
IHC-p 1:200 - 1:1000
ICC 1:200 - 1:1000
FCM 1:200 - 1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using CD1A mAb against human CD1A (AA: 17-116) recombinant protein. (Expected MW is 39.7 kDa)
Western blot analysis using CD1A mouse mAb against Hela (1), HepG2 (2), HEK293 (3),MOLT4 (4),K562 (5),HEK293-6e (6),Cos-7 (7),and NIH/3T3 (8) cell lysate.
Flow cytometric analysis of MOLT4 cells using CD1A mouse mAb (green) and negative control (red).
Flow cytometric analysis of HL-60 cells using CD1A mouse mAb (green) and negative control (red).
Flow cytometric analysis of Jukrat cells using CD1A mouse mAb (green) and negative control (red).
Flow cytometric analysis of K562 cells using CD1A mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD1A mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using CD1A mouse mAb with DAB staining.
Immunofluorescence analysis of Hela cells using CD1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

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