CCL27 Monoclonal Antibody

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Home > Product > Antibody > CCL27 Monoclonal Antibody

CCL27 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: IHC,ICC,FCM

Product overview

Code

BT-MCA2427

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CCL27 (AA: 25-112) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes. This cytokine may also play a role in mediating homing of lymphocytes to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of a similar murine protein indicate that these protein-receptor interactions have a pivotal role in T cell-mediated skin inflammation. [provided by RefSeq, Sep 2014]

Uniprot accession

Q9Y4X3:;

Molecular weight

12.6kDa

Gene ID

10850

Synonyms

ALP;ILC;CTAK;CTACK;PESKY;ESKINE;SCYA27

Research area

Immunology

Target protein

CCL27

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using CCL27 mAb against human CCL27 (AA: 25-112) recombinant protein. (Expected MW is 36 kDa)

Western blot analysis using CCL27 mAb against HEK293-6e (1) and CCL27 (AA: 25-112)-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis of HepG2 cells using CCL27 mouse mAb (green) and negative control (red).

Flow cytometric analysis of Hela cells using CCL27 mouse mAb (green) and negative control (red).

Flow cytometric analysis of A431 cells using CCL27 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using CCL27 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CCL27 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using CCL27 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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