EPCAM Monoclonal Antibody

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Home > Product > Antibody > EPCAM Monoclonal Antibody

EPCAM Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,FCM

Product overview

Code

BT-MCA4756

Host

Mouse

Isotype

Mouse IgG2a

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human EPCAM (AA: extra(116-265)) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Uniprot accession

P16422:;

Molecular weight

35kDa

Gene ID

4072

Synonyms

ESA;KSA;M4S1;MK-1;DIAR5;EGP-2;EGP40;KS1/4;MIC18;TROP1;EGP314;HNPCC8;TACSTD1

Research area

Tags & Cell Markers

Target protein

EPCAM

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200-1:1000
FCM	1:200-1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western blot analysis using EPCAM mAb against human EPCAM (AA: extra(116-265)) recombinant protein. (Expected MW is 38 kDa)

Western blot analysis using EPCAM mAb against HEK293-6e (1) and EPCAM (AA: extra(116-265))-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using EPCAM mouse mAb against HCT116 (1), HT-29 (2),SW480 (3),Sw-620 (4) , and T47D (5) cell lysate.

Flow cytometric analysis of Lovo cells using EPCAM mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EPCAM mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EPCAM mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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