MSH6 Monoclonal Antibody

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Home > Product > Antibody > MSH6 Monoclonal Antibody

MSH6 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA4404

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human MSH6 (AA: 374-540) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described.

Uniprot accession

P52701:;

Molecular weight

152.8kDa

Gene ID

2956

Synonyms

GTBP;HSAP;p160;GTMBP;HNPCC5

Research area

Epigenetics and Nuclear Signaling

Target protein

MSH6

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using MSH6 mAb against human MSH6 (AA: 374-540) recombinant protein. (Expected MW is 22.6 kDa)

Western blot analysis using MSH6 mAb against HEK293-6e (1) and MSH6 (AA: 374-540)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using MSH6 mouse mAb against SH-SY5Y (1), Hela (2), PC-3 (3), HCT116 (4), HEK293 (5), A549 (6), and HepG2 (7) cell lysate.

Flow cytometric analysis of Hela cells using MSH6 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded colon tissues using MSH6 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded brain tumor tissues using MSH6 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using MSH6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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