NEFH Monoclonal Antibody

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Blood coagulation
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Home > Product > Antibody > NEFH Monoclonal Antibody

NEFH Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: IHC,FCM

Product overview

Code

BT-MCA4389

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human NEFH (AA: 2-251) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Uniprot accession

P12036:;

Molecular weight

112.4kDa

Gene ID

4744

Synonyms

NFH;CMT2CC

Research area

Neuroscience

Target protein

NEFH

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200-1:1000
FCM	1:200-1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western blot analysis using NEFH mAb against human NEFH (AA: 2-251) recombinant protein. (Expected MW is 29.6 kDa)

Western blot analysis using NEFH mAb against HEK293-6e (1) and NEFH (AA: 2-251)-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis of SK-N-SH cells using NEFH mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded human cerebrum tissues using NEFH mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using NEFH mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded human medulla oblongata tissues using NEFH mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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