KRT18 Monoclonal Antibody

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Blood coagulation
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Home > Product > Antibody > KRT18 Monoclonal Antibody

KRT18 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA3788

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human KRT18 (AA: 1-150) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Uniprot accession

P05783:;

Molecular weight

48kDa

Gene ID

3875

Synonyms

K18;CK-18;CYK18

Research area

Tags & Cell Markers

Target protein

KRT18

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:500 - 1:2000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using KRT18 mAb against human KRT18 (AA: 1-150) recombinant protein. (Expected MW is 42.1 kDa)

Western blot analysis using KRT18 mAb against HEK293 (1) and KRT18 (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using KRT18 mouse mAb against SK-BR-3 (1), HepG2 (2), T47D (3), MCF-7 (4), Hela (5), A431 (6), and HCT116 (7) cell lysate.

Immunofluorescence analysis of Hela cells using KRT18 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using KRT18 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using KRT18 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using KRT18 mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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