CD177 Monoclonal Antibody

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Home > Product > Antibody > CD177 Monoclonal Antibody

CD177 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,FCM

Product overview

Code

BT-MCA2208

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CD177 (AA: extra 22-161) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified.

Uniprot accession

Q8N6Q3:;

Molecular weight

46.4kDa

Gene ID

57126

Synonyms

NB1;PRV1;HNA2A;PRV-1;HNA-2a;NB1 GP

Research area

Immunology

Target protein

CD177

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using CD177 mAb against human CD177 (AA: extra 22-161) recombinant protein. (Expected MW is 41.3 kDa)

Western blot analysis using CD177 mAb against HEK293 (1) and CD177 (AA: extra 22-161)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using CD177 mouse mAb against SPC-A-1 (1), SK-MES-1 (2), HepG2 (3), HL-60 (4), and PC-3 (5) cell lysate.

Flow cytometric analysis of HL-60 cells using CD177 mouse mAb (green) and negative control (red).

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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