CD322 Monoclonal Antibody

Product

OneStep ELISA kit
Primacu ELISA kit
Antibodies
Biochemicals
Recombinant proteins

Service

Custom ELISA assay services
Bulk quote

Research area

Blood coagulation
Metabolism
Cancer
Microbiology
Cardiovascular
Neuroscience
Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
Tags & cell markers
Immunology

Resources

Protocols
FAQs
Product brochure
Promotion
Troubleshooting guides
About ELISAs

About us

Company profile
Worldwide distributors
Manufacturing service

Home > Product > Antibody > CD322 Monoclonal Antibody

CD322 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,ICC,FCM

Product overview

Code

BT-MCA3462

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CD322 (AA: extra 29-238) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene.

Uniprot accession

P57087:;

Molecular weight

33.2kDa

Gene ID

58494

Synonyms

JAM2;JAMB;JAM-B;VEJAM;PRO245;VE-JAM;C21orf43

Research area

Others

Target protein

CD322

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:100
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using CD322 mAb against human CD322 (AA: extra 29-238) recombinant protein. (Expected MW is 53.3 kDa)

Western blot analysis using CD322 mAb against HEK293 (1) and CD322 (AA: extra 29-238)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using CD322 mouse mAb against NIH/3T3 (1), Ramos (2), and HepG2 (3) cell lysate.

Flow cytometric analysis of HL-60 cells using CD322 mouse mAb (green) and negative control (red).

Immunofluorescence analysis of Hela cells using CD322 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

Customer reviews and Q&As

No reviews

Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

We are continuously updating our Antibody catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.