SHC1 Monoclonal Antibody

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Home > Product > Antibody > SHC1 Monoclonal Antibody

SHC1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse

Applications: WB,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse

Applications: WB,ICC,FCM

Product overview

Code

BT-MCA3526

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human SHC1 (AA: 385-495) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

P29353:;

Molecular weight

62.8kDa

Gene ID

6464

Synonyms

SHC;SHCA

Research area

Neuroscience

Target protein

SHC1

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using SHC1 mAb against human SHC1 (AA: 385-495) recombinant protein. (Expected MW is 37.3 kDa)

Western blot analysis using SHC1 mAb against HEK293 (1) and SHC1 (AA: 385-495)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using SHC1 mouse mAb against NIH/3T3 cell lysate.

Immunofluorescence analysis of A431 cells using SHC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of NIH/3T3 cells using SHC1 mouse mAb (green) and negative control (red).

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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