RPS6KA2 Monoclonal Antibody

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Home > Product > Antibody > RPS6KA2 Monoclonal Antibody

RPS6KA2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA4350

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human RPS6KA2 (AA: 415-734) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Uniprot accession

Q15349:;

Molecular weight

83.2kDa

Gene ID

6196

Synonyms

RSK;HU-2;RSK3;p90-RSK3;pp90RSK3;MAPKAPK1C;S6K-alpha;S6K-alpha2

Research area

Epigenetics and Nuclear Signaling

Target protein

RPS6KA2

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using RPS6KA2 mAb against human RPS6KA2 (AA: 415-734) recombinant protein. (Expected MW is 62 kDa)

Western blot analysis using RPS6KA2 mAb against HEK293 (1) and RPS6KA2 (AA: 415-734)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using RPS6KA2 mouse mAb against Hela (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.

Immunofluorescence analysis of A431 cells using RPS6KA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using RPS6KA2 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RPS6KA2 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using RPS6KA2 mouse mAb with DAB staining.

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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